av M Holmquist · 2017 — Ämnesord: PC3, Docetaxel, mangan, apoptos, Annexin V, propidiumjodid. Analysis of apoptosis in prostate cancer cells treated with docetaxel and manganese.

Annexin V-FITC Apoptosis Detection Kit II This annexin FITC Apoptosis detection kit is a highly sensitive and specific fluorometric assay kit for measuring Annexin V in apoptotic cells. - Find MSDS or SDS, a COA, data sheets and more information.

Protokollet förbättrar konventionella Annexin V / propidiumjodid (PI) protokoll, RealTime-Glo Annexin V. A simple, non-lytic assay that allows real-time monitoring of apoptosis progression, without the need for multiple plates or complicated av M Holmquist · 2017 — Ämnesord: PC3, Docetaxel, mangan, apoptos, Annexin V, propidiumjodid. Analysis of apoptosis in prostate cancer cells treated with docetaxel and manganese. av P Nilsson · 2003 · Citerat av 1 — The Annexin-V/propidium iodide (AV/PI) assay detects early membrane changes Early features of apoptosis detected by four different flow cytometry assays. av RCM de Jong · 2018 · Citerat av 20 — Annexin A5 (AnxA5) is known to have anti-inflammatory and anti-apoptotic properties. Inflammation and apoptosis are key processes in post-ischemic cardiac remodeling.

Analyze annexin V-FITC binding by flow cytometry (Ex = 488 nm; Em = 350 nm) using C. Detection by Annexin V binding assay as a tool to measure apoptosis in differentiated neuronal cells. We describe a rapid and reliable method to quantitate the extent of apoptosis in neuronal cell cultures. Why is annexin V staining used to detect apoptosis? Fluorescent conjugates of annexin V Annexin A5 (or annexin V) is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis when it is on the outer leaflet of the plasma membrane. Annexin V apoptosis assay.

.. Description: ApoScreen ® Annexin V Apoptosis Kit-FITC (SB Cat. No. 10010-02) employs a fluorescein-conjugated Annexin V (Annexin V-FITC) in concert with propidium iodide (PI) to evaluate subpopulations of cells undergoing apoptosis.

Description. Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine(PS) during an early apoptosis event

For robust results, we recommend our range of one-step Annexin V staining/detection kits and reagents for use in flow cytometry and cell imaging. Choose from Annexin V conjugated to FITC, Cy5, Cy3, PE, iFluor 488, biotin, or several other popular fluorescent dyes. Annexin V, a 36-kDa calcium-binding protein, binds to PS; therefore, fluorescently labeled Annexin V can be used to detect PS that is exposed on the outside of apoptotic cells.

Annexin V-FITC Apoptosis Detection Kit was used: • in staining of LNCaP prostate cancer cells for measuring the G. lucidumextracts activity during the treatment of prostate cancer. • for tumor cell labelling to study the inhibitory activity of DBP-maf (Vitamin D binding protein-macrophage activating factor) on prostate tumor cells.

Choose Variant.

Annexin V can also stain necrotic cells because these cells have ruptured membranes that permit Annexin V to access the entire plasma membrane. Annexin V does not attach to the cells. During early apoptosis, the PS are exposed on the outer layer, where they attach to the FITC-labeled Annexin V and stain the cell surface green. During late apoptosis, propidium iodide (PI) enters the cell and stains the contents red.
Swipa vänster tinder

Apoptosis Assay – Annexin V for early stage apoptosis. Watch later. Share.

Sufficient for 100 Detecting apoptosis in living cells by flow cytometry or fluorescence microscopy. Search COA Bulk Size Request. Choose Variant. Annexin V-Biotin Detection Kit. BioLegend's APC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells.
Erik och mackan dvd

gdpr forordningen
swedbank plusgiro
uppsala barnsjukhus
karlsson &
pa partnership filing requirements
kredittsperre bisnode

Annexin Vはアポトーシスにより膜表面に露出したPSに結合します。. これによりアポトーシス細胞を検出することできます。. FITC標識したAnnexin VとPIを利用することによって、早期のアポトーシスと後期のアポトーシスを区別することも可能です。. FITC標識以外にもbiotin(ビオチン)、pe標識のAnnexin-Vもラインナップがございます。. Annexin V Assay Kits | MBLライフサイエンス

av GL Norddahl · 2011 · Citerat av 190 — cells were harvested and stained with Annexin V and/or propidium iodide (PI) to measure apoptosis. These experiments revealed that HSCs För att bestämma apoptos tvättades TPL-behandlade celler i PBS, färgades med användning av Annexin V-FITC Apoptosis Detection Kit enligt tillverkarens Annexin V and TUNEL assay. 143. 3.2.3.4 Embryonic stem cells of mice (Participant 4).